Microbicidal activities: standard protocols
Disinfectants and antiseptics

Surface quantitative test

The aim of these tests was to determine the bactericidal*, fungicidal/yeasticidal** and sporicidal*** activities of chemical disinfectant or antiseptics on a carrier test. For that, at minimum three different concentrations to include one concentration in the active range and one concentration in the non-active range shall be evaluate under mandatory conditions of the standard.

Non-porous surface quantitative assay without mechanical action

MEDICAL Domain

*NF EN 14561 Instruments (mar.2007): P.aeruginosa, S.aureus and E.hirae
Obligatory conditions: contact time (60 min) and 1 clean or dirty condition
The product shall demonstrate at least a 5 decimal log (lg) reduction.
**NF EN 14562 Instruments (sep.2006): C.albicans and A.brasiliensis
Obligatory conditions: contact time (60 min) and 1 clean or dirty condition
The product shall demonstrate at least a 4 decimal log (lg) reduction.



Veterinary domain

*NF EN 14349 (dec.2012): P.aeruginosa, S.aureus, E.hirae and P.vulgaris
Obligatory conditions: 1 temperature (10°C), 1 contact time (30 min) and dirty condition (low or high)
The product shall demonstrate at least a 4 decimal log (lg) reduction.
**NF EN 16438 (mar.2014):  C.albicans and A.brasiliensis
Obligatory conditions: 1 temperature (10°C), 1 contact time (30 min) and dirty condition (low or high)
The product shall demonstrate at least a 3 decimal log (lg) reduction.



INDUSTRIAL DOMAIN

*NF EN 13697 (jun.2015): P.aeruginosa, S.aureus, E.coli and E.hirae
Obligatory conditions: 1 contact time (5 min) and 1 clean or dirty condition
The product shall demonstrate at least a 4 decimal log (lg) reduction.
**NF EN 13697 (jun.2015): C.albicans and A.brasiliensis
Obligatory conditions: 1 contact time (5 min) and 1 clean or dirty condition
The product shall demonstrate at least a 3 decimal log (lg) reduction.



Porous surface quantitative assay without mechanical action

Veterinary domain

*NF EN 16437 (mar.2014): P.aeruginosa, S.aureus, E.hirae and P.vulgaris 
Obligatory conditions: 1 temperature (10°C), 1 contact time (60 min) and dirty condition (low or high)
The result shall demonstrate at least a 4 decimal log (lg) reduction.





Additional conditions according to standards:

- Modification of the strain*
- Additional contact time
- Additional clean and dirty conditions
*Partners